Sequence Analysis of Functional Regions of Homoserine Dehydrogenase Genes froml-Lysine andl-Threonine-producing Mutants ofBrevibacterium lactofermentum
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چکیده
منابع مشابه
Lysine and threonine biosynthesis in sorghum seeds: characterisation of aspartate kinase and homoserine dehydrogenase isoenzymes
Aspartate kinase (AK, EC 2.7.2.4) and homoserine dehydrogenase (HSDH, EC 1.1.1.3) have been partially purified and characterised from immature sorghum seeds. Two peaks of AK activity were eluted by anion-exchange chromatography [diethylaminoethyl (DEAE)-Sephacel] with 183 and 262 mM KCl, and both activities were inhibited by lysine. Similarly, two peaks of HSDH activity were eluted with 145 and...
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Amy C. Schroeder, Chuanmei Zhu, Srinivasa Rao Yanamadala, Rebecca E. Cahoon, Kiani A. J. Arkus, Leia Wachsstock, Jeremy Bleeke, Hari B. Krishnan, and Joseph M. Jez From the Department of Biology, Washington University, St. Louis, Missouri 63130, the Donald Danforth Plant Science Center, St. Louis, Missouri 63132, and the Plant Genetics Research Unit, United States Department of Agriculture-Agri...
متن کاملEffect of inducible thrB expression on amino acid production in Corynebacterium lactofermentum ATCC 21799.
Amplification of the operon homdr-thrB encoding a feedback-insensitive homoserine dehydrogenase and a wild-type homoserine kinase in a Corynebacterium lactofermentum lysine-producing strain resulted in both homoserine and threonine accumulation, with some residual lysine production. A plasmid enabling separate transcriptional control of each gene was constructed to determine the effect of vario...
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The nucleotide sequence of the Serratia marcescens threonine operon (thrA1A2BC) was determined. Three long open reading frames were identified; these open reading frames code for aspartokinase I (AKI)-homoserine dehydrogenase I (HDI), homoserine kinase, and threonine synthase, in that order. The predicted amino acid sequences of these enzymes were similar to the amino acid sequences of the corr...
متن کاملAnalysis of strain-specific genes in glutamic acid-producing Corynebacterium glutamicum ssp. lactofermentum AJ 1511.
Strains of the bacterium, Corynebacterium glutamicum, are widely used for the industrial production of L-glutamic acid and various other substances. C. glutamicum ssp. lactofermentum AJ 1511, formerly classified as Brevibacterium lactofermentum, and the closely related C. glutamicum ATCC 13032 have been used as industrial strains for more than 50 years. We determined the whole genome sequence o...
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ژورنال
عنوان ژورنال: Bioscience, Biotechnology, and Biochemistry
سال: 1997
ISSN: 0916-8451,1347-6947
DOI: 10.1271/bbb.61.1760